FastQCFastQC Report
Tue 15 Mar 2016
40-FW17B3Muscle_131203_L004_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename40-FW17B3Muscle_131203_L004_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17229408
Sequences flagged as poor quality0
Sequence length10-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCG50950.049.1638394
CCACGTA55150.040.6152652
CACGTAT64500.036.863653
ATACGCC32000.034.2131847
GCCCCGT15300.032.2430271
ACTGGCC53700.031.42361594
CGTTAGG42650.031.1687245
CGCTACG49950.030.65600634-35
TACGGGG49750.029.76113738-39
CCCGCTA51900.029.75452232-33
CCGCGTA11250.029.6287292
GACCCGC15000.029.19460794
CTACGGG52600.028.7374436-37
GCTACGG53150.028.31195436-37
ACGTATT88900.028.29564
TCCACGT84250.028.0110171
GTACCTA96500.027.2102857
GTCGCGT39050.026.85718784-85
ACGTTAG42700.026.5409584
GGGGCGC48400.026.41883976-77