FastQCFastQC Report
Tue 15 Mar 2016
4-Wingbud8A1_131203_L002_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4-Wingbud8A1_131203_L002_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23217896
Sequences flagged as poor quality0
Sequence length10-100
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA40900.025.2852761
CCCCCGT30050.023.4848481
TCGGTCC55750.023.01948288-89
GCCCCGT27950.022.5497461
CGGTCCT56900.022.36745588-89
CCCCGTT51400.021.7606832
AGGCCGA55400.021.74910436-37
AAGGCCG54900.021.74091136-37
GGCCCCT36650.021.5566431
GGGCCGT23100.021.135661
CCCCGTA30000.020.7129462
CCCCGAA55500.020.7129462
AGTCGGT61250.020.69607586-87
GGGGGTT55000.020.6591471
GTCGGTC61300.020.50864686-87
GGGGGGT33650.020.3128451
CAGTCGG64250.019.56591284-85
CCCGAAT55100.019.4133533
GGGGGTG48350.019.1860221
GCCCGTA26850.019.0102842