FastQCFastQC Report
Tue 15 Mar 2016
4-Wingbud8A1_131203_L002_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4-Wingbud8A1_131203_L002_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336165
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGT400.00286632633.80454682-83
GAGCGCG801.8193768E-631.8352269
TAGTCGC350.00593181229.13830858-59
TCTCCCG1000.005978788229.08039588-89
AATCGGA1200.0090838426.6905394
TGAGCGC1151.0718377E-625.2910798
AGGGACC901.5057987E-424.1938957
TAGCCAA1051.5644595E-524.1398721
CGCTATC650.001892264823.2016374-75
AAATCGG1202.3181314E-522.94263692-93
ATGCGGG952.203241E-422.8727595
GGACCGT800.002186213922.6311321
CGGGGGT850.003036713521.385848
AAGGCCC1054.3382737E-420.6913191
GTGAGCG1255.907923E-520.322877
GGGACCA1105.777424E-419.8305058
AGCGCGT657.762744E-519.61878410-11
GGGGGTA950.00575666919.1490089
GGTCTCC950.006309718418.84738286-87
GTCGGTC900.00644167518.78030282-83