FastQCFastQC Report
Tue 15 Mar 2016
38-Normal8A3Ovary_131121_L008_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename38-Normal8A3Ovary_131121_L008_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24262153
Sequences flagged as poor quality0
Sequence length10-100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT38700.029.4542751
CCACGTA83550.028.5311812
ACCTAAG188950.027.3886189
CTACGGG54050.027.08037636-37
CCCCGTG30750.026.4981942
TACGGGG54650.026.37896338-39
CGCTACG56550.025.96777534-35
GCTACGG56650.025.68114536-37
CACGTAT95650.025.6470133
GTACCTA201700.025.5114147
CTACGCG28600.024.5917669
ACGGGGC60450.024.32567438-39
CCCGCTA60750.024.29547132-33
ACCGGGT50300.024.15681584-85
CGGTACC137300.023.9365985
CCGCCCG61300.023.84070290-91
GGGGCGC60000.023.62187276-77
AAGCGCC60500.023.52734866-67
TCCCTCG105600.022.9020211
GACCGTC67200.022.85446414-15