FastQCFastQC Report
Tue 15 Mar 2016
37-Normal8A3Muscle_131121_L008_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename37-Normal8A3Muscle_131121_L008_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30002776
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCG109600.036.7946794
CCACGTA108000.030.0503852
CACGTAT114250.029.1794573
CTACGGG74800.028.89996136-37
GTACCTA164450.027.5796957
TACGGGG78450.026.81290238-39
ACCTAAG170400.026.4030599
CGCTACG84050.025.82809834-35
GCTACGG83150.025.72698836-37
CGGTACC116350.024.9047225
GGCCGTT88200.024.5809881
GACCCGC32050.024.30500494
CCCGCTA91050.023.96891432-33
CCGGTAC113950.023.366354
CCCCCGA38200.023.118151
AAGCGCC90300.022.49540566-67
GACCGTC99350.022.49198314-15
GGGGCGC86500.022.46184276-77
GGCTTAC96600.022.29758846-47
TAGGGAC75350.021.90525636-37