FastQCFastQC Report
Tue 15 Mar 2016
37-Normal8A3Muscle_131121_L008_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename37-Normal8A3Muscle_131121_L008_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30002776
Sequences flagged as poor quality0
Sequence length10-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCCATGGCCTGCACGCGCTTCATCTGGGTTTTCGCCGCCGTGGCCG1552410.5174221212063843No Hit
AGGCCGGGGAAACTCATCACCGCTCGCGTGCACGCTCGAGCTACCCGACC438090.1460164886075875No Hit
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG437930.14596316020890868No Hit
ATGCCGCAATGGGCCCCTGCTCTCGACTTGACTGCCAACGCGACGCTGGT390190.13005129925310913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCT212700.078.7081394
TTCAGCC268850.056.6432231
ATGGCCT284700.053.3561558
CCATGGC302000.050.385136
AGCCATG309750.049.3229984
CAGCCAT316000.048.6309433
CATGGCC320850.047.46877
TCAGCCA336600.045.82772
GCCATGG338950.045.3396225
TGGCCTG340150.044.7127729
ATCGCGG207450.037.16012264-65
GGCGGTC237250.035.5523892-93
ATGGGCG229750.035.38408790-91
GCGCGCA240150.033.7853484-85
CGCGGCT231100.033.5181866-67
TGGGCGG246800.032.90665490-91
GCATGGG245450.032.78276488-89
CGCGCTT235950.032.6593318-19
CCGCAAT119450.032.4346354
CGCGCAT246950.032.40395484-85