FastQCFastQC Report
Tue 15 Mar 2016
35-Normal21A2Ovary_131121_L008_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename35-Normal21A2Ovary_131121_L008_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30140953
Sequences flagged as poor quality0
Sequence length10-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA33650.028.503741
CCCCGTG29600.026.832152
CCCCCGT38550.026.6820161
CCCGCCG93400.026.66831894
CCCCGTA35000.025.6683772
CTACGGG48700.024.77328736-37
TACGGGG47450.024.71010438-39
GCTACGG49600.023.78721836-37
CGCTACG51800.023.6931834-35
ACCTAAG173050.023.6114229
GGGCCGT28000.023.4052661
CCGCCCG52450.023.2609490-91
CCACGTA88650.023.2057552
GCCCCGT37800.023.1928561
GGGGCGC50550.023.11207876-77
CGGTACC120150.023.0897245
GGGGGTT74000.022.228091
ACGGGGC55300.022.04730638-39
GTACCTA187650.021.7380757
CCCGCTA56750.021.5725832-33