FastQCFastQC Report
Tue 15 Mar 2016
35-Normal21A2Ovary_131121_L008_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename35-Normal21A2Ovary_131121_L008_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30140953
Sequences flagged as poor quality0
Sequence length10-100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGA71950.033.718456
ACGACCG72800.033.5036055
CGGACCA79800.033.3083845
ACCATCG96100.032.44529394
ACCGAGC74950.032.1375588
CGCAACG64600.031.7222371
GACCGAG79150.031.039577
CCGAGCG82850.029.3923059
CGGATCG48400.029.08067386-87
AACACGG96700.028.2558631
GACGACC85950.028.1727814
GGCCAGG114600.027.78874694
ATCGGGC57250.025.8506190-91
CATGACG99200.025.2925971
ACACGGA111100.025.1059022
AGCCGGG72400.025.00958674-75
CGCGGTT78250.024.15938886-87
GCGGATC59500.023.84107486-87
GGATCGG61100.023.3069388-89
GATCGGG64400.022.1563388-89