FastQCFastQC Report
Tue 15 Mar 2016
35-Normal21A2Ovary_131121_L008_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename35-Normal21A2Ovary_131121_L008_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286218
Sequences flagged as poor quality0
Sequence length10-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACACGGACCAAGGAGTCTAACATGTGCGCGAGTCAATGGGAGCTTCTAA11910.4161163868100538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATCG1650.0104.90382494
CGGTTGA1950.056.5075488-89
CGACCGA750.055.462326
CGCGGTT1950.055.06387786-87
AATCGGA703.4150586E-452.0575494
GTTGACC2300.049.67232590-91
GACCGAG850.049.0405277
AGCCGGG2650.047.23843474-75
GCGGCGC2400.046.6070682-83
CCGGGCG2700.046.4198976-77
AAATCGG801.6773669E-545.55034694
GAAGCCG2900.044.2149872-73
GGGCGCG2450.044.13134878-79
ACGACCG950.043.7170525
TGACCAT2950.042.24145592-93
ACCGAGC953.6379788E-1240.2855458
GCGCGGC2900.038.47818480-81
CCGAGCG1007.2759576E-1238.3590289
GGTTGAC2150.036.06553688-89
GCGGTTG2100.035.98089286-87