FastQCFastQC Report
Tue 15 Mar 2016
32-Normal6A3Ovary_131203_L005_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename32-Normal6A3Ovary_131203_L005_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20720037
Sequences flagged as poor quality0
Sequence length10-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACC90250.032.5673075
ACCTAAG128800.031.909889
GTACCTA130300.031.6266277
CCCCCGA25500.030.1205221
CCGGTAC87950.030.0584
GCCCGGT64100.029.1897372
CCCGGTA77400.028.674093
GGTACCT123900.027.5521326
GCCCCGT30250.027.3099291
CCCCGAT27000.025.6355232
TACCTAA163250.025.2224438
TACGGGG30200.023.87030438-39
CCCCCGT29350.023.5829331
GGGGGGT29500.023.463021
CCCCGTT50050.023.1976222
CCACGTA56750.022.9768892
GGGGGTT49200.022.9630551
CCCCGTA27500.022.8960532
CCCGGGA104300.022.31679780-81
CGTATCA122800.022.26364392-93