FastQCFastQC Report
Tue 15 Mar 2016
3-Wingbud21B_131203_L002_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3-Wingbud21B_131203_L002_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33357915
Sequences flagged as poor quality0
Sequence length10-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC185950.032.8064466
GTGTGCT254950.032.3665127
TGTGCTC277000.029.7715288
GTGCTCT275150.029.7477769
CCCCCGA48500.027.7068711
GCCCCGT35450.024.3334881
GGGTGTG138300.023.9564785
CCGATCT196650.023.3852116-17
GGGCCGT30750.023.1188531
CCCCGTA42200.021.6739232
CCCCGAT41500.020.9949822
CGGTGTG87700.020.7188785
GGCCCCT47950.020.6116981
TTCCGAT236500.019.88838414-15
GCCCCGA32300.019.8621711
GACCCGA24500.019.816161
CCCCGAA76800.019.8113212
TCCGATC190650.019.39982816-17
GGGGGTT78450.019.2288741
GGGGGGT47200.019.0106031