FastQCFastQC Report
Tue 15 Mar 2016
3-Wingbud21B_131203_L002_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3-Wingbud21B_131203_L002_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389584
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCA855.820766E-1141.0549245
ACACGGA1206.730261E-1131.9804082
AATCGGA1652.5263831E-531.4294394
AAATCGG1956.548362E-1131.0264994
CATGCGC450.00437321431.0113071
AACACGG1202.3719622E-929.07311
CTTAGCC500.007318494427.9101751
TACCACG657.771672E-427.0276789
GGCGCGT808.785778E-526.1927096
CACGGAC1358.367351E-925.83283
ACGGACC1401.2372766E-824.9006044
AGCCGGG700.00217654822.65013174-75
GCGTGGC952.704896E-422.1911459
CCGGGGA952.852667E-422.0173784
ACCAAGG1656.6695065E-821.2423158
TGGGCGC1004.034241E-420.9165084
GAAGCCG904.426401E-420.63179672-73
CGACCGA1205.801986E-520.3721076
GGCGCAA1059.195481E-619.74684358-59
CCGAGCG1257.579947E-519.676159