FastQCFastQC Report
Tue 15 Mar 2016
29-FW23B1Testis_131203_L005_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename29-FW23B1Testis_131203_L005_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155498
Sequences flagged as poor quality0
Sequence length10-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGT3320.21350756922918623No Hit
AGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTT2220.1427671095448173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACG300.00683634552.6903162-63
CCACCGG304.9577735E-448.2824975
GTCCCGA250.002794670433.97630336-37
ACCTAAG552.3823802E-433.058169
CCTGCAC450.003637579332.1883281
CGTTACC300.004782666530.44471444-45
GATCGGT500.00599492829.063018
GGCGTGC500.00605190429.0068327
GTACCTA656.449228E-427.8911847
TAAGTCG406.54533E-527.34808712-13
TCCGAGT300.0085778227.01386330-31
CCGGGGA957.1629966E-626.6824324
ACACGTC550.00954534226.4209188
CCAGTTT550.00969656226.3359073
CCGCAAA550.00969656226.3359071
CCTAAGT451.4736532E-424.27009410-11
GCGTGCT750.001469893824.2191758
GCCGGGG901.5230753E-424.1412473
AGGCCGG901.5230753E-424.1412471
CTACGGA551.6158643E-524.0377620-21