FastQCFastQC Report
Tue 15 Mar 2016
28-FW23B1Muscle_131203_L005_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename28-FW23B1Muscle_131203_L005_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145281
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG3490.24022411740007293No Hit
CGCAACGATGATTATTTTCTACTAATCATAAAGACATTGGAACTTTATAT2150.14798906945849769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCAA250.00100222185.49818488-89
GCAGAGC552.3771226E-455.99102894
ATGACGT200.005842169754.83580890-91
GTGCGAT200.005866112754.7791752
CGCTATC551.5825208E-1049.22035274-75
AGCTGGG304.7542027E-448.69261
TCGGTCT507.216295E-848.01959284-85
CGTCGGT551.8852916E-742.49995882-83
TTCGCTA659.586074E-1040.9627672-73
CACACGT456.9889575E-540.717337
CCATTCG554.9737337E-639.83943
ACTAGCT708.185452E-1139.76796766-67
TCCCGCA653.434361E-739.36929390-91
AGACTAG701.05501385E-1038.8504864-65
GGTCTCC654.7570393E-737.76849486-87
CACGTCT501.2867074E-436.7089929
ACCGTGA400.001958396836.519453
GGTTCAG400.001958396836.519451
AGCCGGG350.002636502334.3761274-75
AAGGCGC451.6108172E-533.57512756-57