FastQCFastQC Report
Tue 15 Mar 2016
27-FW23B1Head_131203_L005_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename27-FW23B1Head_131203_L005_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17324645
Sequences flagged as poor quality0
Sequence length10-100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGT751790.4339425136849846No Hit
AGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTT748000.4317548786713956No Hit
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG601380.34712399590294635No Hit
TGAAACCCAAAGAATTTGGCGGTGTTTTAGTCCTTTCAGAGGAATCTGTC173910.10038300929109947No Hit
TTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGG173830.1003368322987282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGAGG208000.031.9164543
GCCGGGG50450.031.7317523
CCGGGGA52250.030.119044
AGGAGGT209350.029.5304497
GAGGTGA210450.029.4849919
GCAGGAG229350.027.7865332
TGCAGGA229300.027.733421
GGAGGTG226350.027.5127648
CAGCTTA87500.026.824994
AGGCCGG62850.025.5473141
ACCAGGC154700.025.31561778-79
GTCATCC149150.024.89741750-51
GGTGACC147350.024.80134410-11
GCGGTCT22600.024.00479594
CAGGCTT164750.023.97676380-81
CTCTACC153150.023.90154622-23
GGCCGGG70300.023.483332
TGACCCT159000.022.81540912-13
CGCAACG35400.022.7425371
GACCCTA140750.022.45053314-15