FastQCFastQC Report
Tue 15 Mar 2016
26-FW17B2Testis_131203_L005_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26-FW17B2Testis_131203_L005_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28215093
Sequences flagged as poor quality0
Sequence length10-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCT321200.1138397807159452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG112500.041.5180669
CGAGAAT152000.031.8055029
GGGGGGG75200.031.4200571
AACGAGA156350.030.6176017
GGGGGGT69500.030.1842122
TTTACGC156900.030.0126518
GGGGGGA66900.028.5846441
GGTTTAC153650.028.5580566
CCCATAG100000.027.77284694
CGGTACC81100.027.454185
CAGTTCG190300.027.39565894
AGTTCGC115800.027.23983694
GTACCTA121250.027.2319977
GTTTACG176200.026.8422227
CGCTCTA75250.026.64653494
CCCGCCG98400.026.21709894
ACGAGAA185850.026.003718
GGGGGTA61350.025.4835381
GGGTTTA171700.025.3948525
GCCCGGT62550.025.2064652