FastQCFastQC Report
Tue 15 Mar 2016
24-FW17B2Head_131203_L004_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename24-FW17B2Head_131203_L004_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232006
Sequences flagged as poor quality0
Sequence length10-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGA602.3995384E-455.9084194
CGCACGG200.006441072653.5095569
GTACCCC350.001181163740.4877172
TGCCAGG400.002276042535.426752
AAATCGG1000.002966142733.54504494
GAGTCTA451.3713543E-731.99785614-15
CAGGCTT752.1327924E-631.18765480-81
TAATGGG500.005105365530.03942588-89
CGGACCA604.6374605E-429.5159265
CGGGTCG250.006424406528.666112-13
GTTAGAT500.00667850728.433427
CCCAGCC500.00678510428.3414021
CACGGCC456.5260765E-427.84611956-57
CGCTGAG657.444083E-427.2219814
CCGCTGA657.444083E-427.2219813
ACACGGA700.001140125225.3048252
CTCGTCG1001.9939192E-523.39074180-81
AGGCGCA652.0401577E-423.133756-57
TCGTCGG750.002394260122.27689680-81
AACACGG800.002479991222.141721