FastQCFastQC Report
Tue 15 Mar 2016
23-FW11B3Testis_131203_L004_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename23-FW11B3Testis_131203_L004_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271763
Sequences flagged as poor quality0
Sequence length10-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT3820.14056365288873024No Hit
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG3720.13688397611153835No Hit
AGGCCGGGGAAACTCATCACCGCTCGCGTGCACGCTCGAGCTACCCGACC2860.1052387558276881No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT2720.10008720833961945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGA855.0367817E-967.2652394
GCGGTCT400.003766418461.25940794
TAGCCCC350.001159319740.64421
CACGTCT458.037279E-539.7787259
CGGGGCT951.5825208E-1037.428564
AAATCGG1003.243258E-936.17464492-93
TACCCCT400.00223404635.5636751
ATCGCGG401.8480093E-434.53413464-65
CCCGGGG1054.6020432E-1033.8701632
TCCTAAC450.003292796432.85777388-89
ACGTACG651.8833338E-532.8219684
AACACGG552.720181E-432.3306121
CGCGCAT450.00427805631.1478684-85
GGGGCTC1151.20599E-930.9648235
CACCGAT350.004412112830.9545362-63
CTACGTA703.1214156E-530.4831492
GGGCTCC1301.3278623E-1030.1700696
GCGCATG950.00510998430.02571786-87
GGCTCCC1352.0190782E-1029.106367
CGTACGA754.9539965E-528.4876355