FastQCFastQC Report
Tue 15 Mar 2016
22-FW11B3Muscle_131203_L004_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename22-FW11B3Muscle_131203_L004_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213136
Sequences flagged as poor quality0
Sequence length10-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCCTCCACAGGCAATCACAATGGGTCGCTCTCTCACTTTCCTGTTG2310.1083815028901734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCATC400.001993556571.8965694
CGGGTAC150.00277739966.172688
GCAGAGC500.00482680557.5172594
CGGTTGA401.4700792E-553.01881488-89
AATCGGA753.72514E-451.12644294
CTTAGGC200.00891001149.291821
AATAGCG355.199649E-542.82488666-67
CGCGGTT506.497751E-541.22887886-87
AGCCGGG656.9990347E-735.96278474-75
GCCCTTA601.8661174E-532.8612142
AGGTCCT601.8661174E-532.8612142
GTTGACC703.2287923E-431.38968790-91
CCACTTA653.2248998E-530.3334312
GTCGGTC704.6940957E-429.449286-87
GACCGAG450.00569445729.3753877
CCAGGGT250.005805324829.26566524-25
TGACCAT804.9332954E-429.19748592-93
CACTTAT705.3462958E-528.1667563
GCGGTTA300.00735060927.88402736-37
AGCCGAC906.941542E-626.79736176-77