FastQCFastQC Report
Tue 15 Mar 2016
21-FW11B3Head_131203_L004_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename21-FW11B3Head_131203_L004_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228561
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG3200.14000638779144298No Hit
CGCAACGATGATTATTTTCTACTAATCATAAAGACATTGGAACTTTATAT2690.11769286973718177No Hit
AGGCCGGGGAAACTCATCACCGCTCGCGTGCACGCTCGAGCTACCCGACC2400.10500479084358223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGA602.4243728E-455.79081794
CGGACCA352.0247615E-550.2368935
GGATCGG450.00830160350.16463588-89
CGCGGTT450.00913160348.97023886-87
CCGTCGC252.91334E-547.24679648-49
GAGCGCG404.348551E-544.141129
CGACCGA404.434042E-543.995866
CGCTATC453.6283036E-641.72883274-75
CCGAGCG458.7097775E-539.2365579
GCCTACC458.996107E-539.0218161
CGTCGGT556.770213E-638.10379482-83
CTCCCGC607.056906E-637.87216688-89
TCCCGCA651.0098611E-535.94320790-91
ACCGAGC501.6255012E-435.2895978
GACCGAG501.6360456E-435.2508247
CGATGAT904.2382453E-935.1966866
TGACGAC501.6787907E-435.096593
TCGCTAT551.6583466E-533.4372872-73
TTCAGCC1206.184564E-1132.1929971
GACGACC552.938127E-431.9059874