FastQCFastQC Report
Tue 15 Mar 2016
2-Wingbud21A1_131203_L002_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2-Wingbud21A1_131203_L002_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25601393
Sequences flagged as poor quality0
Sequence length10-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA30000.030.8600541
GCCCCGT26950.026.6273461
GGCCCCT37000.025.0216641
CCCCCGT34300.023.891821
AGGGTGC89450.023.5905256-57
CCCCGTT57700.023.261542
GGGGGGT36050.023.2235261
CCCCGTA27350.023.160632
GGGCCCT36450.023.0902021
CCCCGAT30950.021.8978982
CCTTAGG98300.021.85792222-23
TACCGAC34000.021.7915577
GAGGGGC99200.021.6306134-35
CCGCACC121050.021.23878160-61
GGGGGTT65850.020.8534341
ACCGACC38100.020.1553828
GCCCGTA26650.019.9459932
ACAGGGT107600.019.85812254-55
GGCCTTA109950.019.82169520-21
CTTAGGG103750.019.61066222-23