FastQCFastQC Report
Tue 15 Mar 2016
2-Wingbud21A1_131203_L002_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2-Wingbud21A1_131203_L002_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335729
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGA605.381002E-447.48867884-85
CGCGAAT750.001355278839.36353786-87
CGTCGGT400.00210690435.9930384-85
CCGCGAA400.00210690435.9930384-85
CGGGGGT450.00413368431.3690158
GGACCGT1101.0117546E-625.4590421
GACCGTG1253.0765004E-622.4039572
CAGACGG800.002667434521.8690913
CGAGTCA605.5304857E-419.96316330-31
TCCAGCG950.00719289618.4243072
TGGCTAT1150.001007822818.2559343
CGTTTGC1000.008235848517.99651384-85
GGCAACC1000.00966666917.5030921
AGCACCT1000.0096915817.4952724
CCCAGAT1000.0096915817.4952724
ACCGTGA1602.604556E-517.4952723
CGAGCTC955.208076E-616.86075412-13
CCGGGGA1250.001762271616.795464
AGTCTGG1300.002234016816.2098396
TACCTTA1300.002290390716.149484