FastQCFastQC Report
Tue 15 Mar 2016
18-Normal8A2Head_131203_L004_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename18-Normal8A2Head_131203_L004_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248495
Sequences flagged as poor quality0
Sequence length10-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCGGGGAAACTCATCACCGCTCGCGTGCACGCTCGAGCTACCCGACC2730.10986136541982737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGA306.267285E-446.0513843
ACCATCG1004.5311564E-543.8034794
AATCCGC358.333784E-443.46098786-87
AGTACGT503.8013313E-641.4462471
CATCGCC400.001177011940.5151190-91
GACCATC858.223378E-735.22825692-93
CGGACCA806.92562E-834.5733265
GCGCGGT651.4539784E-534.08354684-85
GGGCGCG601.6217253E-533.5461378-79
TCCCGCA851.2580513E-633.36538390-91
GCGGTTG701.9730973E-532.5957486-87
AACACGG751.6478443E-632.2359731
TCGGCAC1100.003819721531.8570794
ATATACG403.2134075E-431.42799654-55
GCAGAGC1702.8139833E-530.92009794
TGCCTCG450.004590651530.7009242
CGGGCGC653.4063843E-530.09504576-77
GAAACCG703.675823E-529.760188
TCGGTCT1002.7542592E-729.53907284-85
TGCGCGT350.00569492929.37978256-57