FastQCFastQC Report
Tue 15 Mar 2016
15-Normal21A1Head_131203_L003_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15-Normal21A1Head_131203_L003_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22253685
Sequences flagged as poor quality0
Sequence length10-100
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGT917490.41228677407809083No Hit
AGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTT876000.39364267086552185No Hit
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG741790.33333355801522313No Hit
TGAAACCCAAAGAATTTGGCGGTGTTTTAGTCCTTTCAGAGGAATCTGTC363440.16331677203123887No Hit
AGCTGGTATACCACGACGGTACTCCGATTATCCAGACGCTTATACTTCAT223320.10035191924393647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTTA137150.031.10509394
CAGGAGG261100.030.843783
AGGAGGT268900.027.6188417
GCAGGAG292250.026.9171492
GAGGTGA280900.026.8929969
TGCAGGA295200.026.435341
CCGGGGA46000.025.8323524
CGCAACG51650.025.4418891
GCCGGGG46800.025.2945063
ACCAGGC196450.025.15377478-79
GTCATCC184200.025.0196650-51
GGTGACC179700.024.89161510-11
CAGGCTT206700.023.9739580-81
CTCTACC190700.023.84813322-23
GGAGGTG320100.023.8113578
TGACCCT196900.022.76968412-13
CCAGGCT194800.022.16818680-81
GAAACCC158250.021.9356882
CCTATTC208000.021.7140716-17
CGACCGA45750.021.703096