FastQCFastQC Report
Tue 15 Mar 2016
14-Normal6A1Testis_131203_L003_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename14-Normal6A1Testis_131203_L003_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239578
Sequences flagged as poor quality0
Sequence length10-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGGG951.10958354E-1038.6736379
ATGCTCC1053.7107384E-1034.5521432
CCCCAGG1105.4205884E-1033.3576478
CTCCCCC1105.6024874E-1033.259335
TGCTCCC1106.1299943E-1032.974713
GCTCCCC1106.1299943E-1032.974714
GAGCCCG800.003753370631.98012580-81
CCCCCAG1252.1464075E-929.3361477
TCCCCCA1303.3069227E-928.1721656
AGGCCGG1303.6579877E-927.90751
AACACGG656.4375007E-427.90751
CTTGTCG450.00823102527.24358276-77
GTCCTCC550.00962844726.3797664
GGTCCTC550.00962844726.3797663
CCCCCGC550.00962844726.3797663
GCCGTGA550.00962844726.3797664
TGGGTCG1550.001306801124.70606694
GAGGTCC750.001483858224.18651
CTAGGCC901.5071702E-424.18652
CGTCCGT1852.5465852E-1023.5278973