FastQCFastQC Report
Tue 15 Mar 2016
11-Wingbud23B2_131203_L003_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename11-Wingbud23B2_131203_L003_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278601
Sequences flagged as poor quality0
Sequence length10-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGA1001.881177E-645.8853194
AACACGG608.254958E-637.022451
AAATCGG1301.1407632E-535.29639494
ACACGGA651.4286834E-534.174572
CTTGCCG450.003268203532.908841
AGCCGGG400.005527966729.5595774-75
TGAGCGC805.4258482E-528.1100718
ACACTCG550.00871615426.9254154
CAAATCG1051.13240585E-525.1651492-93
CTAGGCC750.00131917124.6816311
CGGACCA800.001830245423.3317495
GAGCGCG1002.4485512E-422.5167089
GCCGGGG1002.6872134E-422.2094783
CCGGGAG900.0036266420.7393325
CCGTCGA500.003886698720.49686830-31
CCCACAA1105.1087263E-420.1940611
GAGTCTA757.963008E-620.0788114-15
GCCCACG1000.00699730218.5112232
GTGTGCC1050.00863622917.8476648
ACGGACC1050.00926978117.6297364