FastQCFastQC Report
Tue 15 Mar 2016
10-Wingbud23B1_131203_L003_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename10-Wingbud23B1_131203_L003_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18371918
Sequences flagged as poor quality0
Sequence length10-100
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA28250.031.136281
CGCCACG34650.029.34392494
CCCCGAT26150.025.4823552
GCCCCGT22400.025.1869561
CCCCGTA25950.023.624452
CGGTACC34150.021.2115755
GCCCGGT29100.021.0671632
GGGGGTA34400.021.0498561
GGTCCGA16900.021.0292241
TCCCCGT37750.020.8293781
GGGGGGT26100.020.7653541
CCCCGAA44850.020.6025452
GGGCCGT16400.020.5868421
CCCCGTT44250.020.3799322
CCCGGTA36350.020.165063
GGGGGTT42850.020.1127221
CCCGAAT49600.019.9729943
CCGGTAC37950.019.7831254
ATAATCG65850.019.77487294
GACCCGA13750.019.385121