FastQCFastQC Report
Tue 15 Mar 2016
10-Wingbud23B1_131203_L003_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename10-Wingbud23B1_131203_L003_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223285
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGT253.1826436E-452.83296682-83
TCGGTCT350.001395679839.1369984-85
CGCTATC350.00277217834.03278774-75
CTGGGTC552.1475312E-433.655518
CCGGGGA1008.039933E-932.9606864
TGGGTCT753.762036E-529.6571439
GCCGGGG1002.9579314E-729.2983883
AGGCGGA500.005768616729.2983883
CGGAGAC655.809374E-428.4005626
GGTCTCC500.007168737728.02486-87
GACAGCG550.00917576126.6408652
TCCCGCA550.00930685926.5634990-91
AAGGGGG708.747526E-426.4795919
GCGGGGG708.864042E-426.4196837
GTCGGTC500.00956399526.41648382-83
ACGCTCG501.3050131E-424.71117432-33
ATGCGGG750.001348463924.586065
CACCGCT701.5926526E-724.2467418-19
CGGGGAA1551.9963409E-823.7929615
CGAGCTA450.001748935923.51760936-37