FastQCFastQC Report
Tue 15 Mar 2016
1-Wingbud6A2_131203_L002_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1-Wingbud6A2_131203_L002_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20827273
Sequences flagged as poor quality0
Sequence length10-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCG72300.029.38021594
CCACGTA67800.028.9364912
CACGTAT77950.026.8088233
CCCCGTA25350.026.087282
TACGGGG53900.025.1899438-39
CTACGGG54700.025.09821136-37
GCCCGTA22200.023.8310852
TCGGTCC47100.023.50588288-89
CGCTACG59250.023.35013434-35
CCCCCGA32350.022.8955211
TACCGAC32200.022.4875457
GCTACGG60600.022.46865736-37
CCCGCTA63550.021.86640732-33
GGGCCCT26200.021.7072071
GCCCCGT25350.021.3915711
ACCTAAG106900.021.2499739
ACCGACC33700.021.2347938
GCGGGCT61850.021.2298344-45
GGGGGGT32850.021.204951
ACGGGGC64050.021.12754838-39