FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11198668
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT10950.08.52784449
CGGTACA13900.07.70414261
GGCGGTA13601.8189894E-127.380933359
GTTAGAT29500.06.81379461
GCGGTAC15303.6379788E-126.772709460
AATCGTC11251.8852006E-86.73081975
GATTCCG13402.6375346E-106.72349352
TCTCGAT13402.764864E-106.710219448
GGAAAAT107350.06.682691
GTTTATT70250.06.61060951
ATATCGG7854.945042E-56.576994
GTTAGAC17209.094947E-126.24620631
GTCTAAG19050.06.161424644
GTTTAAT54150.06.1440651
GAATCGT14903.5615813E-96.0061014
CCCGAAT12701.7945968E-76.00346571
GTAGTCG8802.1344669E-45.91363757
TCGATTC14103.8224243E-85.888260450
GTTTTAT91050.05.8617031
GTTACAT44400.05.85411641