FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11198668
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4701.2732926E-1113.2128131
CGACCGT5750.012.59942232
GTCGGTT9850.011.9478821
GTCCGTT13700.011.874781
GTCCGTA13400.011.3656981
GTCGTAT11400.011.2342771
GTTCGAC5958.240022E-1010.43700929
GTCCGCT12950.010.4242091
GTTCGTT18350.010.3746841
TCGCGAG3702.9517876E-510.2564742
GATCGCG3552.1953703E-49.71816940
TCGACGC8052.0008883E-119.42830843
GCCCTTT48850.09.4240021
CCCGATT9550.09.423631
GTCGCGT5151.2080927E-69.40955451
GGCGTTT18400.09.4059021
GTCGGTA11500.09.3306541
GCCGTTT18600.09.3047621
GTTCGAT15050.09.1996591
GTCCAAT34900.09.1245341