FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10406265
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG16850.09.01306844
CTCGATT12950.08.01098949
GGCGGTA16500.07.760581559
CGGTACA17150.07.65330861
ATTCCGT14250.07.29632853
TCTCGAT14151.8189894E-127.086575548
GCGGTAC19100.07.052177460
GATTCCG15250.07.04238452
TTCTCGA15151.8189894E-126.82855947
TCAATCG12706.4574124E-106.7766853
CAATCGG10603.0917727E-76.49557644
TGGGACG29600.06.4368841
TCGATTC15603.274181E-116.429873550
TTCGATC8107.612477E-56.3751042
TAGCATA28050.06.2591639
CGAAAAT13651.8670107E-86.0909261
CAATCGA7450.0012435166.0073184
GTCCGAT6350.0091442386.00100041
CTAAGTA25200.05.889627546
CCGGACA21750.05.85599312