FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10406265
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT12350.011.7656631
GTCCGCT13300.011.7056331
GTCGAGA7101.8189894E-1210.7200891
CCGGTAT9400.010.6734351
CTCCGTT26250.010.5437411
GTCGCAT7550.010.5393771
CGCTAGT3301.0207583E-410.4838331
GTCGGTT8750.010.28014851
TCGACCG6051.1004886E-910.26443131
GCCGTAT10300.010.07669451
CCCGAAT12750.010.0398121
CGAATCT12400.010.0183123
TCCGTTA10550.09.8152542
GCGGTAT9250.09.7244631
CGATCTA4653.4936547E-69.646434
CTCGTAT16350.09.5220141
CGACCGT6554.3437467E-99.48070532
CTCCGAT20000.09.3410951
CTCCTAT30750.09.1132641
CCGTCTA3804.322872E-49.0834162