FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19304576
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT23300.09.79428849
TCGATTC27350.08.34579150
GATTCCG27800.08.22540452
TCTCGAT26800.08.12766448
GGCGGTA29900.07.87191759
TTCTCGA29150.07.80818947
CGGTACA29100.07.59712761
TCACCCG38050.07.5086353
ATTCCGT30250.07.448409653
CGCTAGT9151.2201508E-66.787546633
GTCTAAG35650.06.777387644
GTTTAAT91150.06.7265451
CGATTCC36100.06.718156351
CCGGACA39500.06.622577712
GCGGTAC34950.06.522684660
CCCGGAC43800.06.52245611
GTTCAAT52350.06.41845561
GTCGATT10803.8774306E-76.41477971
GGAAAAT182400.06.32404381
ATTCTCG19700.06.29064566