FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19304576
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG11800.016.6652231
CGACCGT13750.014.30119732
GTTCGAC14850.012.31310729
GCGATCG13650.011.87894440
CGATCGG13950.011.37564541
CCCGTAT18600.011.3460881
TTCGACC17800.011.2415530
TCGCGTA8300.011.2215966
TCGACGC16950.010.99081543
GTCCGAT19450.010.4944981
CCCGAAT21650.010.3868711
GTCGGTT19550.010.2638551
CGCGTAA8800.010.19205367
GTCCGCT25150.010.17941951
GTCGCTT27700.09.9917011
AGTTCGA19550.09.88233328
GTCCGTT23650.09.8010571
AAGTTCG20500.09.76080527
GTTCGAT23300.09.5028381
GTCCGGT27500.09.4353471