FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9967688
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT11450.010.56969749
ATTCCGT14250.09.48477353
TCTCGAT13450.08.99735948
GATTCCG14350.08.6905252
TTCTCGA14350.08.41161847
GGCGGTA15500.08.26276759
GACGTAA7252.2723907E-78.08338341
AATCCCG8951.6407284E-98.077215
TCGATTC16050.07.973423550
GCGGTAC18050.07.84700860
CGGTACA17500.07.6993661
GTCTAAG18800.07.52770944
CGTGGGT21750.07.17667557
TCACCCG20200.07.1574933
GAATCGT14203.6379788E-127.03042174
GTTCAAT26350.06.96947531
CGATTCC19900.06.96316251
GTTTAAT48500.06.7871381
ACGGGCG21800.06.682799356
GTTTGAT59900.06.5945151