FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9967688
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG7100.014.57745631
CGACCGT7650.013.52879432
GTCCGCT12000.013.2623851
GTTCGAC8100.012.7777729
CCCGTAT9000.012.6857591
GTCGCTT13750.012.0776821
GTCGGTT9400.011.7778811
GTCGAGA6109.094947E-1211.34345151
TCGACGC10050.011.32801143
GTCCGTT11900.011.0479491
TTCGACC9850.010.857861530
CTCGTAT13550.010.7239561
GTCGTTT17800.010.6902461
GCCCGTT19550.010.6181671
GTCCGGA12700.010.3520161
TCTCGAC11550.010.15543241
GTCGGTA9250.010.0987371
GTCCGAA10400.09.9800561
GTCCGTA11600.09.84241
CTCGGAT14850.09.7851591