FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4620560
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT4701.2732926E-1113.24304649
TCGCGAA2450.001095284911.26674333
TAATCGC4653.5040455E-58.93606759
CGGGAAT8252.910383E-108.8194931
TCGATTC6302.2106724E-78.78475550
TTCTCGA6808.081224E-88.619740547
GCGTAGT4007.39255E-48.60732823
GATTCCG6101.2788332E-68.51935352
TTGCGTA4058.3566364E-48.50180121
CGATACG5806.5044787E-68.30791629
ACTAGGC7051.4890975E-78.298779511
TCTCGAT6302.087574E-68.23284248
GTTTTCG4300.00151787348.00430410
CAACTAG8851.1361408E-87.77819359
ACGGAAT6251.7089576E-57.728985333
TCGTTGA6755.4228767E-67.698970352
CGGTACA6301.863164E-57.67897661
TATTCGC4050.0079215567.64839177
GGTTGCG4550.0026466157.56426119
TCACCCG8706.916525E-87.5165233