FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4620560
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGA3408.424322E-712.2122481
GTCGAAT4256.6229404E-912.2122481
CCGGTAT6201.8189894E-1211.7198191
CCGAATT3954.2781357E-711.3591872
CGACCGT2500.001298555311.03960532
GTCGGTT3207.458126E-510.8129281
GTCGTTT8650.010.8004271
CGAATTG3207.667947E-510.7834333
CCGGCTA3602.1797985E-510.54197537
GTCGATT7051.2732926E-1110.3067911
GGCGTAT5805.7207217E-910.1417811
GTCGGAC4451.921111E-610.1082651
GTCCAAT13500.09.9959511
GTCGGAA7657.2759576E-129.9507211
TCCGATA5654.3210093E-89.7686155
GTCCAGT21850.09.6598791
GTCCGAT6453.1632226E-99.6561971
GGCCTAT6151.4930265E-89.5646061
GCGTATA2900.0044358639.5212292
CCGATAG5806.472692E-89.51628356