FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22089522
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAT104600.07.51833441
CTCGATT24600.07.17009649
CGGTACA31300.06.842533661
GTTTATT143550.06.8057091
ACTAGGC27900.06.78546810
GGCGGTA30850.06.732546359
GATTCCG27350.06.33448652
GTTTTTT279150.06.31695271
CGATACG21350.06.126631729
GTTAAAT100900.06.11161951
TCTCGAT28650.06.035339448
GTTTTAT178300.06.00391631
TTCTCGA33450.05.979800747
TCGATTC29000.05.964357450
ATTCCGT29900.05.912726453
GTTCAAT61700.05.8956491
GTAGTCG20701.8189894E-125.865857
GGAAAAT201850.05.85265731
CGGGAAT38050.05.82710461
GGGGAAT80500.05.8098561