FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5950143
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA7750.012.03711661
GGCGGTA8550.010.9325159
TAGCATA15450.08.6889879
GCGGTAC9951.8189894E-128.68012560
GTCTAAG10407.2759576E-128.29705444
TCTCGAT7655.89298E-88.13671348
AATCGGG4250.00135102098.0993735
GAAATCG11851.8189894E-127.84293173
ACGGGCG12302.6375346E-107.050751756
CGTATAT5500.00216025426.89066440
ATTCTCG7058.652783E-56.8358716
CGAGAGT5250.0099755046.59861851
CTCGATT7351.4039314E-46.587196449
GGGCGGT13201.3496901E-96.556689358
ACACGGG14301.72804E-106.54453754
GGGTAAT11351.3958925E-76.40967131
CACGGGC15201.10958354E-106.387827455
GTTAAAT30750.06.30892371
AATCCAA32200.06.30719765
AAATCGA9351.2758657E-56.2587124