FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5950143
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCT8450.013.1013511
GTCGCTT8700.012.7248741
GTCGGAT7250.012.4067531
CTATCCG2902.8179358E-511.8960848
GTACGAT5753.6379788E-1111.431641
GTCGGCT8200.011.3912841
GTCGAAT4904.50018E-911.2965721
CCCGTAT5452.059096E-910.7913351
CCCGTAC7101.8189894E-1210.719811
CTCCGAT11950.010.4221511
GGCCGTT9700.010.343061
GTCGTTT11350.010.0586771
CCCGAAT6553.8926373E-1010.0354091
GCCAGTT30850.09.9806211
GTCGAGT5201.2951932E-79.9795441
GTCCGCA8850.09.7728111
CCGAATC7202.237357E-109.5871462
GGCGTTT9100.09.5043271
GCCGTAT5857.070412E-89.4620861
CCGTAAT6251.9481377E-89.4100441