FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4791542
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA2950.0049427799.3971021
GACTCGA4109.460605E-48.3961794
CGACAAT3850.00473282358.1004241
GTTAAAT24750.07.98041771
CTTCAAT22600.07.666331
GTTGAAT17800.07.5922511
GTTTAAT23300.07.43601131
GGCGGTA5250.00125549137.253369359
CAATCGG4800.0044538157.1717364
ACGGGCG8402.1229898E-67.021719556
CGGTACA6159.1622054E-46.73987561
GGATAAC14101.2369128E-106.6354531
CGGGAAT7854.5020162E-56.62136551
CCCGGAC8552.3202478E-56.441308511
GTTTTAT37050.06.35984751
GTTTTTT61800.06.33601141
TCCGCGC6100.00598170846.251774354
GTTCAAC16652.5465852E-116.24357031
GTTTATT30700.06.2079791
TTCAACG8904.169243E-56.1883217