FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4791542
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGA3301.4915713E-1015.7282051
GTCGTAT4101.8189894E-1215.1911431
CCCGATT4600.014.29215051
CCCTAGT3303.977766E-813.631111
GTCGTTT9050.012.9996651
GTCGCGT2153.5676823E-412.8751811
CCCGTAT3801.8260835E-812.7481241
GTTCGGT5750.012.6372691
GTCGCTA3357.0403985E-712.3947631
GCGACTA2255.3464615E-412.2719942
CTCCGAT8000.011.6781921
GTCCGGA6251.4551915E-1111.0726551
CCGCATT4702.9333023E-811.0432071
GGCGACT4157.8348967E-710.8391961
GCCGTTT8350.010.774291
CTCGACT4501.9098661E-710.7650821
GTCGGGA5855.820766E-1010.6467851
GCCGTAT4251.0645235E-610.5841571
CCCGCAT6553.45608E-1110.5655121
GTCGCAT4301.2372129E-610.4610851