FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16920263
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT14600.014.2191961
CGCGTAT2751.0451404E-613.8400191
TCGACCG7800.012.38495831
GTTCGCG4501.1787051E-912.3022381
GTCCGAT22050.011.4548911
GTCGCAT13400.011.3612091
GATCGCG7251.8189894E-1210.46879240
GTCCGCT19800.010.3101151
CGATCGG10950.010.08166741
CGACCGT9300.010.01584332
GTCCGAA16350.09.7345691
GTCGGCA34500.09.7281281
GTCGAGA11900.09.5949711
CCCGAAT21200.09.3028421
TCGCGTA7051.5606929E-99.29675666
GTCCAAT52000.09.1157811
TCGCGAG8851.4551915E-118.96595342
GTCGTTT29700.08.8538841
GTCGGAT24300.08.8279951
GGCGTTT26800.08.7791161