FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21780719
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA31400.07.481312361
CCGCTTA18000.06.932568653
ATCGACT34500.06.88413146
GTTTATT138150.06.7712561
GGCGGTA34200.06.478165659
ATTCCGT30100.06.448953
CTCGATT25750.06.446606649
ATCGGAT33200.06.42795376
TCGACTG28550.06.3894577
GGAAAAT198600.06.38495971
GTTTAAT104200.06.3174561
AATCGGC28950.06.20024946
ACTAGGC28350.06.191378611
GTTAAAT100300.06.14858871
TCTCGAT27700.06.117195648
GTCTAAG39550.06.1094944
TCGTTGA28400.06.1000651
CAATCGG37850.06.09294
CGCTAGT10953.562809E-65.98663733
GATTCCG29000.05.97382452