FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21780719
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG9850.015.06086231
TCGCGTA7550.013.70701966
CGACCGT11900.012.17586932
CGCGTAA8950.011.5630167
GTCCGAT28950.011.2344041
GTCGGTT19600.011.1212911
CCGGTAT22350.010.8365551
GTCGTTT35350.010.7664861
GTTCGAC14150.010.72787929
TCGACGC15400.010.08092643
GTCCGTT29050.010.0046961
CCCGAAT26350.09.8480761
GTCGCGA5704.730464E-89.7121611
GTCGCTT31750.09.6987771
GTCGGGA34600.09.5998821
GTCCGCT29000.09.42541
GTCCAGT100350.09.4127391
GTCGTAT22200.09.3512361
GTCGCAT16300.09.3397631
GTCGAGA17050.09.3347831