FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5049950
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG6251.9033723E-78.87118152
CTCGATT5857.1784416E-78.86833349
TCGATTC5908.082625E-78.79562850
TTCTCGA7901.2030796E-88.29631147
TCTCGAT7402.9925286E-77.945230548
GGCGGTA7453.2908974E-77.898115659
TCACCCG9607.748895E-107.88714273
TTTCTCG9454.4638E-97.671917446
CGGTACA7251.8960563E-67.62400661
CGATTCC7402.4618494E-67.492551351
ACCCGGC10152.397428E-97.4599825
ATTCCGT7452.7071146E-67.445235353
CAGTCGA5754.4312948E-47.1828134
CGGACAC11657.1304385E-107.08941813
TTCGGTG8352.1944616E-67.00697662
GTCTAAG8008.324016E-66.904862444
CCGGACA10151.4151374E-76.78119712
GTTCGAT5200.0090984536.66192251
CCCGGAC9906.602677E-76.60481811
CGGAGAT10007.0097667E-76.58197931