FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5049950
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT5950.015.6998541
TCGACCG2201.6227423E-615.68221231
GTCGAAA3403.8417056E-914.2461631
CGACCGT2557.5110547E-613.5286832
CGCTAGT1550.00796502113.3927071
GTCGCGT2952.3021057E-612.9008831
GTCGGTT6000.012.6858691
GTTCGAC3104.1393505E-612.24224429
CCCGTAT5207.4578566E-1111.9761711
GTCGTAT5258.731149E-1111.8621111
GTCCGAG7950.011.7502041
GGCCCGT5955.456968E-1211.6295211
GTCGTAA4207.369454E-811.5326081
GTCGTGA4853.8271537E-911.4137151
GTCGCCT4904.494723E-911.2972491
GCCTTAT10600.011.0974151
GTCGATT4753.434252E-810.9256291
GTCGGAT5401.7662387E-910.8919071
CCGGTAT4157.847557E-710.83787251
GTTCGAT6201.4370016E-1010.602561