FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590925
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG2000.003098958412.075767535
CTCGAAC3308.515979E-611.475056
AATAGCG3702.4161454E-611.189935
CGCGGTA2550.001560444610.79922417
CGATAAT2700.0023746510.2678241
CGAACGA3854.6778587E-59.8355448
CTCGATT5755.4312422E-68.420114549
ATAGCGT4653.555712E-48.16180936
GCGTATA3800.00445113948.15581339
GCGGTAC6858.07313E-78.06789160
TCGAACA4000.007077417.74532137
CGGTACA6402.306617E-57.55659561
TAGCGTA4150.00964062857.481994637
CGATTCA4650.00326721377.40317734
CGTATAT5200.00119169777.289071640
TCGATTC6805.051728E-57.12166450
TCTCGAT6855.5865166E-57.067437648
GATTCCG6401.8190556E-47.036836652
GGATAAT12403.237801E-106.98667431
GTTCAAT13756.730261E-116.8047661